Separation and identification of urinary constituents by use of multiple-analytical techniques.

High-pressure anion-exchange chromatography with gradient elution has been used to resolve ultraviolet-absorbing constituents from normal and pathologic specimens of human urine. We have identified 50 of these constituents by using multiple-analytical techniques. Ultraviolet spectral maxima, gas chromatographic methylene unit (MU) retention values (for trimethylsilyl derivatives), and the major mass spectral peaks are reported for each of the identified compounds.